Download Aquaculture Genome Technologies by Zhanjiang (John) Liu PDF

By Zhanjiang (John) Liu

Genomics is a swiftly becoming medical box with purposes starting from superior ailment resistance to elevated cost of development. Aquaculture Genome applied sciences comprehensively covers the sector of genomics and its purposes to the aquaculture undefined. This quantity appears to be like to bridge the distance among a easy realizing of genomic know-how to its sensible use within the aquaculture undefined.

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However, 500,000 fragments are too many to be resolved in any kind of gel electrophoresis. This demands that somehow the 500,000 bands must be reduced approximately 2,000 times to reach the resolvable goal of a couple hundred bands. Vos and others (1995) intelligently met this challenge by adding additional arbitrary bases at the 3Ј end of the PCR primers. As each extra arbitrary base is added, the PCR primer can match to only 1/4 subset of the fragments because at each base of DNA, and there are four possibilities: A, C, G, or T.

In a welldefined closed mating system involving limited number of founders, genetic mapping using AFLP can add much greater resolution to framework linkage maps made with microsatellites. Acknowledgments Research in my laboratory is supported by grants from the USDA NRI Animal Genome and Genetic Mechanisms Program, the USDA NRI Basic Genome Reagents and Tools Program, the Mississippi-Alabama Sea Grant Consortium, the Alabama Department of Conservation, the USAID, and the BARD. References Agresti JJ, S Seki, A Cnaani, S Poompuang, EM Hallerman, N Umiel, G Hulata, GAE Gall, and B May.

239–244. Borowsky R and H Wilkens. 2002. Mapping a cave fish genome: polygenic systems and regressive evolution. J Hered, 93, pp. 19–21. Crossland S, D Coates, J Grahame, and PJ Mill. 1993. Use of random amplified polymorphic DNAs (RAPDs) in separating two sibling species of Littorina. Mar Ecol Prog Ser, 96, pp. 301–305. Dinesh KR, WK Chan, TM Lim, and VPE Phang. 1995. RAPD markers in fishes: an evaluation of resolution and reproducibility. Asia-Pac J Mol Biol Biotechnol, 3, pp. 112–118. Hirschfeld D, AK Dhar, K Rask, and A Alcivar-Warren.

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